Antje Reiss1, Inge S Fomsgaard2, Solvejg K Mathiassen3 and Per Kudsk4
Forsøgsvej 1. 4200 Slagelse. Aarhus University, Department of Agroecology. Denmark
The lack of non-destructive sampling methods that allow for repeated sampling of secondary plant metabolites in the root zone is restricting the understanding of allelopathic interactions in intact plant communities. Here, we determine the robustness of silicone tube microextraction (STME) to technical and environmental effects, as well as its applicability under outdoor conditions. Sampling volume, change of solvent, sampling speed, temperature and soil water content were identified as factors with significant influence on the recovery of the benzoxazinoids (BX) benzoxazolin-2-one (BOA) and 6-methoxy-benzoxazolin-2-one (MBOA) and the phenoxazinones 2-acetylamino-3H-phenoxazin-3-one (APO) and 2-amino-7-methoxy-3H-phenoxazin-3-one (AMPO). The STME method was successfully applied under outdoor conditions and detected a stable concentration of BOA and a steady increase of its degradation products APO and AMPO released by winter wheat, winter triticale and winter rye grown in styrofoam boxes. Silicone tube microextraction was shown to be a suitable, easily applicable and cheap method for repeated sampling of benzoxazinoids in the root zone of winter cereals throughout their growing season.
Keywords: Secondary plant metabolite, cereals, wheat, triticale, rye, soil.
RECEIVED: February 25, 2018
ACCEPTED: May 23, 2018